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Mouse Anti-Human HSP70 Monoclonal IgG, Clone C92F3A-5

  • Catalog number
    SMC-100B-FITC
  • Product name
    Mouse Anti-Human HSP70 Monoclonal IgG, Clone C92F3A-5
  • Size
    200 µg
  • Price
    446EUR
  • Supplier
    StressMark antibodies
  • Ask
  • Clone
    C92F3A-5
  • Immunogen
    Human HSP70
  • Antibody's full description
    Mouse Anti-Human HSP70 Monoclonal IgG Antibody, Clone: C92F3A-5: FITC
  • Antibody's category
    Monoclonal Antibodies
  • Antibody's other name
    HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
  • Raised in
    Mouse
  • Antibody's target
    HSP70
  • Primary research fields
    Cancer, Heat Shock
  • Brandname
    None
  • Antibodies' applications
    WB, IHC, ICC/IF, ELISA, FCM, FACS, IEM, Bl, AM
  • Antibody's reactivity
    Human, Mouse, Rat, Bovine, Nematode (Caenorhabditis elegans), Dog, Chicken, Fruit Fly (Drosophila melanogaster), Carp (Cypriniformes), Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit, Sheep
  • Antibody's dilutions
    WB (1:1000), IHC (1:10000), ICC/IF (1:1000), FACS (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.1% sodium azide
  • Antibody's concentration
    1 mg/ml
  • Antibody's specificity
    Detects ~70kDa. Does not cross-react with HSC70 (HSP73).
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at more.
  • Bibliography
    1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424. 8. Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879. 9. Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239. 10. Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377. 11. Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248. 12. Dressel et al. (2000) J. Immunol. 164: 2362-2371. 13. Verma A.K., et al. (2007) Fish and Shellfish Immunology. 22(5): 547-555. 14. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
  • Released date
    1-Jan-2007
  • PubMed number
    28052117, 28053100, 27524398, 27509516, 27527204, 27409630, 27354066, 25552722, 25552722, 25355627, 25412999, 25412999, 25412999, 25034784, 25356750, 25356750, 25200073, 24903326, 25038226, 25400510, 25024384, 24705179, 24625088, 24447452, 24447452, 24516376, 23429211, 23598404, 22763123, 22343013, 22496374, 22050377, 21975426, 22010006, 22039833, 22039833, 21737627, 21320889, 21522149, 21522149, 20924639, 21253609, 21253609, 20130532, 19706692, 19056281, 19209902
  • Tested applications
    Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Flow Cytometry, Western Blot, Western Blot, Immunocytochemistry/Immunofluorescence, Western Blot, Western Blot, Immunoprecipitation, Flow Cytometry, Western Blot, Western Blot, Protein Inhibition, Western Blot, Western Blot, Immunoprecipitation, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Electron Microscopy, Immunoprecipitation , Western Blot, Western Blot, Immunohistochemistry, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot, Western Blot
  • Tested reactivity
    Human, Human, Mouse, Human, Human, Human, Human, Human, Human, Human, Bovine, Bovine, Bovine, Human, Mouse, Mouse, Human, Mouse, Mouse, Rat, Human, Rat, Mouse, Human, Human, Mouse, Pig, Human, Rat, Human, Dog, Human , Rat, Pig, Human, Human, Human, Rat, Human, Human, Human, Mouse, Human, Human, Pig, Mouse, Rat
  • NCBI number
    NP_005336.3
  • Gene number
    3303
  • Protein number
    P08107
  • Antibody's datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. Of Oncology, Poland. Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (SMC-100) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida. Mouse Anti-Hsp70 Antibody [C92] used in Immunohistochemistry (IHC) on Mouse colon carcinoma (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Heat Shocked Melanoma cells (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-100) Mouse Anti-Hsp70 Antibody [C92] used in Western Blot (WB) on Human cell lysates from various cell lines (SMC-100) Mouse Anti-Hsp70 Antibody: FITC [C92] used in Fluorescence Activated Cell Sorting (FACS) on Mouse Heat Shocked CD3+ CD8+ T cells (SMC-100)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight (kg)
    1.4
  • Net weight (g)
    0.2
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Additional isotype
    IgG
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Mouse Anti- HSP70 Monoclonal IgG, Clone C92F3A-5
  • Technique
    anti-Human, antibody to, antibodies against human proteins, IgGs, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse antibody to-H. sapiens HSP70 monoclonal Immunoglobulin G, clonality C92F3A-5
  • Alternative technique
    antibodies
  • Clone name
    C92F3A-5
  • Ask
MESH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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