Mouse Anti-Human GABA A Receptor Epsilon Monoclonal IgG2A, Clone S413-67

  • Catalog number
    SMC-493D-HRP
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S413-67
  • Immunogen
    Fusion protein amino acids 27-253 (Extracellular N-terminus) of human GABA A Receptor Epsilon
  • Antibody s full description
    Mouse Anti-Human GABA A Receptor Monoclonal IgG2A Antibody, Clone: S413-67: HRP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Gamma-aminobutyric acid receptor subunit epsilon Antibody, GABRE Antibody
  • Raised in
    Mouse
  • Antibody s target
    GABA A Receptor
  • Primary research fields
    Neuroscience, Ion channels, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Human, Rat
  • Antibody s dilutions
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~60kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:100 dilution of SMC-493 was sufficient for detection of GABA-A R, Epsilon in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Junction , Synapse , Postsynaptic Cell Membrane , Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    The GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Brainstem expression of GABA-A Receptor, Epsilon, is upregulated during pregnancy (3).
  • Bibliography
    1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Hengen K.B., et al. (2012) PLoS One. 7(1): e30608.
  • Released date
    10-Mar-2015
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_004952.2
  • Gene number
    2564
  • Protein number
    P78334
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Epsilon Monoclonal Antibody, Clone S413-67 (SMC-493). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Epsilon Monoclonal Antibody (SMC-493) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Epsilon Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~60 kDa GABA A Receptor Epsilon protein using Mouse Anti-GABA A Receptor Epsilon Monoclonal Antibody, Clone S413-67 (SMC-493). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Epsilon Monoclonal Antibody (SMC-493) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa. Mouse Anti-GABA-A Receptor Epsilon Antibody [S413-67] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-493) Mouse Anti-GABA A Receptor Epsilon Antibody [S413-67] used in Western Blot (WB) on Rat Brain (SMC-493)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    IGHVIII-67-2, IGHVIII-67-3, IGHVII-67-1, IGLV10-67, IGHV1-67, IGHVIII-67-4, ABAT, RNU6-67P, ABHD17B, GOLGA8A
  • Short name
    Mouse Anti- GABA A Receptor Epsilon Monoclonal IgG2A, Clone S413-67
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2A, IgG2a
  • Label
    HRP
  • Species
    Human, Humans, Mouses
  • Alternative name
    Mouse Antibody toHuman GABA A Receptor Epsilon monoclonal IgG2A, clonality S413-67
  • Alternative technique
    murine, antibodies
  • Clone name
    S413-67
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-67-2 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-67-2
    • immunoglobulin heavy variable (III)-67-2 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-67-3 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-67-3
    • immunoglobulin heavy variable (III)-67-3 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (II)-67-1 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (II)-67-1
    • immunoglobulin heavy variable (II)-67-1 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin lambda variable 10-67 (pseudogene)
  • Synonyms gene name
    • immunoglobulin lambda variable 10-67
    • immunoglobulin lambda variable 10-67 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-05-08
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin lambda locus at 22q11.2
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 1-67 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable 1-67
    • immunoglobulin heavy variable 1-67 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-04
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (III)-67-4 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-67-4
    • immunoglobulin heavy variable (III)-67-4 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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