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Clone
12A9
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Immunogen
Purified recombinant fragment of human CD45 expressed in E. Coli
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Antibody s full description
Mouse Anti-Human CD45 Monoclonal IgG1 Antibody, Clone: 12A9: RPE
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
B220 Antibody, CD 45 Antibody, CD45R Antibody, GP180 Antibody, LCA Antibody, Protein tyrosine phosphatase receptor type c polypeptide Antibody, PTPRC_HUMAN Antibody, T200 glycoprotein Antibody
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Raised in
Mouse
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Antibody s target
CD45
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Brandname
None
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Antibodies applications
WB, IHC
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Antibody s reactivity
Human
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Antibody s dilutions
WB (1:1000); optimal dilutions for assays should be determined by the user.
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Antibody buffer for storage
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
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Antibody s specificity
Detects endogenous CD45 proteins. (~147KD).
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:1000 dilution of SMC-256 was sufficient for detection of CD45 in 10 µg of Human HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
CD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, broadly expressed amount hematopoietic cells. There are kmultiple isoforms of CD45 distributed throughout the immune system, and they arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
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Bibliography
1. Barclay A., et al. (1997) The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge I.S., et al. (1993) Annu. Rev. Immunol. 12:85. 3. Kishihara K., et al. (1993) Cell. 74:143. 4. Pulido R., et al. (1988) J. Immunol. 140:3851.
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Released date
06-Sep-2016
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_563578.2
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Gene number
5788
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Protein number
P08575
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Antibody s datasheet
Contact our support service
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Representative figure legend
Western Blot analysis of Human HeLa cell lysates showing detection of ~147 kDa CD45 protein using Mouse Anti-CD45 Monoclonal Antibody, Clone 12A9 (SMC-256). Primary Antibody: Mouse Anti-CD45 Monoclonal Antibody (SMC-256) at 1:1000. Predicted/Observed Size: ~147 kDa. Mouse Anti-CD45 Antibody [12A9] used in Western Blot (WB) on Human HeLa cell lysates (SMC-256)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus