MDM2 Antibody
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Catalog numberR30449
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenMDM2
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting points. Titration of the MDM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis MDM2 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ00987
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PurityAntigen affinity
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DescriptionMDM2 is an important negative regulator of the p53 tumor suppressor. The protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
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ImmunogenAmino acids 343-358 (EKAKLENSTQAEEGFD) were used as the immunogen for this MDM2 antibody.
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StorageAfter reconstitution, the MDM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMDM2
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Short nameAnti-MDM2
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to MDM2
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameMDM2 proto-oncogene
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Synonyms gene name
- mouse double minute 2, human homolog of; p53-binding protein
- Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
- Mdm2 p53 binding protein homolog (mouse)
- MDM2 proto-oncogene, E3 ubiquitin protein ligase
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Synonyms
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Locus
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Discovery year1993-12-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers RANBP2-type
- Ring finger proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data