MDM2 Antibody

  • Catalog number
    R30449
  • Price
    Please ask
  • Size
    0.1mg
  • Category
    Antibody
  • Concentration
    0.5mg/ml if reconstituted with 0.2ml sterile DI water
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    MDM2
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Species reactivity
    Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB
  • Recommended dilutions
    Western blot: 0.5-1ug/ml
  • Notes
    The stated application concentrations are suggested starting points. Titration of the MDM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This MDM2 antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot
    Q00987
  • Purity
    Antigen affinity
  • Description
    MDM2 is an important negative regulator of the p53 tumor suppressor. The protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
  • Immunogen
    Amino acids 343-358 (EKAKLENSTQAEEGFD) were used as the immunogen for this MDM2 antibody.
  • Storage
    After reconstitution, the MDM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
  • Properties
    If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    MDM2  
  • Gene symbol
    MDM2
  • Short name
    Anti-MDM2
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    Antibodies to MDM2
  • Alternative technique
    antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    MDM2 proto-oncogene
  • Synonyms gene name
    • mouse double minute 2, human homolog of; p53-binding protein
    • Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
    • Mdm2 p53 binding protein homolog (mouse)
    • MDM2 proto-oncogene, E3 ubiquitin protein ligase
  • Synonyms
  • Locus
  • Discovery year
    1993-12-10
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Zinc fingers RANBP2-type
    • Ring finger proteins
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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