mCherry Quantification Kit
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Catalog number
K971-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Fluorescence (Ex/Em 587/610 nm) • Species reactivity- Mammalian • Application- It detects a wide range of mCherry (0.01-1 ng/µl)
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Detection Method
Fluorescence (Ex/Em 587/610 nm)
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Species Reactivity
Mammalian
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Applications
It detects a wide range of mCherry (0.01-1 ng/µl)
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Sample Type
Cell and tissue cultures
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Features Benefits
• Simple procedure; takes ~ 20 minutes • Fast and convenient
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
mCherry is the second generation monomeric red fluorescent protein that was derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), such as GFP or DsRed. mCherry is widely used as a fluorescent tracer in transfection and transgenic experiments because of its better performance in brightness, photostability and monomeric structure. Since mCherry requires no additional substrates or cofactors, mCherry can be easily detected under a fluorescence microscope. However, most imaging studies of mCherry are only qualitative. BioVision’s mCherry Quantification Kit provides an easy 96 micro-plate assay to analyze mCherry expression level in cells or tissues quantitatively. Cells or tissues can be homogenized directly in the mCherry Assay Buffer. The amount of mCherry is determined by comparing its fluorescence (Ex/Em= 587/610 nm) with a mCherry standard that is provided in this kit. Each kit provides sufficient reagents to perform up to 100 assays.
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Gene target
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Short name
mCherry Quantification Kit
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Alternative name
mCherry Quantification reagent
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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