MBD4 Antibody

• Catalog number
  R31152
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  MBD4
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the MBD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This MBD4 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O95243
• Purity
  Antigen affinity
• Description
  Methyl-CpG-Binding Domain Protein 4, also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells(Hendrich and Bird, 1998). Riccio et al.(1999) mapped the gene to chromosome 3q21-q22 by FISH. Hendrich and Bird(1998) found that both MBD2 and -4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and -4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al.(1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.
• Immunogen
  An amino acid sequence from the C-terminus of human Methyl-CpG-Binding Domain Protein 4 (YHDWLWENHEKLSLS) was used as the immunogen for this MBD4 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the MBD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MBD4  
• Gene symbol
  MBD4
• Short name
  Anti-MBD4
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to MBD4
• Alternative technique
  antibodies
• Alternative to gene target
  methyl-CpG binding domain protein 4, MED1, MBD4 and IDBG-55837 and ENSG00000129071 and 8930, pyrimidine-specific mismatch base pair DNA N-glycosylase activity, nuclei, Mbd4 and IDBG-179645 and ENSMUSG00000030322 and 17193, BT.49400 and IDBG-646370 and ENSBTAG00000021645 and 511463

Gene info

• Identity
  HGNC:6919
• Gene
  MBD4
• Long gene name
  methyl-CpG binding domain 4, DNA glycosylase
• Synonyms gene name
  • methyl-CpG binding domain protein 4
• Synonyms
  • MED1
• GenBank acession
  • AF072250
• Locus
  3q21.3
• Discovery year
  1999-01-11
• Entrez gene record
  8930
• Pubmed identfication
  • 9774669
  • 10097147
• RefSeq identity
  • NM_003925
• Classification
  • DNA glycosylases
  • Methyl-CpG binding domain containing
• VEGA ID
  OTTHUMG00000159463

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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