LIM kinase 1 + 2 Antibody, Biotin Conjugated

• Catalog number
  bs-11871R-Biotin
• Price
  Please ask
• Size
  0.1ml

• Long name
  LIM kinase 1 + 2 Polyclonal Antibody, Biotin Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Biotin
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  LIM kinase 1 + 2
• Specificity
  This is a highly specific antibody against LIM kinase 1 + 2.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic peptide derived from human LIM kinase 1 + 2
• Tested applications
  WB, IHC-P
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  LIMK 1 and 2 likely regulate aspects of the cytoskeleton, through control of the organization of actin filaments. They can phosphorylate an actin-binding protein, cofilin which binds to actin monomers and polymers and promotes the disassembly of actin filament.The phosphorylation of cofilin via LIMK inactivates this potential. LIMK1 is highly active in the brain and spinal chord, where it is believed to be involved in the development of nerve cells whilst LIMK2 is ubiquitously expressed in many adult tissues. LIMK1 may play an important role in areas of the brain that are responsible for processing visual-spatial information (visuospatial constructive cognition). These parts of the brain are important for visualizing an object as a set of parts and performing tasks such as writing, drawing, constructing models, and assembling puzzles. LIMK1 is specifically stimulated by Rac, one of the Rho family proteins, while LIMK2 activity is activated under the control of other Rho family members, Rho and Cdc42, suggesting that two distinct pathways exist in the Rho family driven actin cytoskeleton dynamics.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  N/A
• Synonyms
  LIM kinase 1+2; LIMK1+LIMK2; LIM domain kinase 1; LIM domain kinase 2; LIMK1; LIMK2;LIMK1_HUMAN; LIMK2_HUMAN.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Additional conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  LIM   kinase   Conjugated  
• Gene symbol
  PDLIM5, LHX1
• Short name
  Anti-LIM kinase 1 + 2
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Biotin
• Alternative name
  Anti-LIM kinase 1 + 2 PAb Biotin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:17468
• Gene
  PDLIM5
• Long gene name
  PDZ and LIM domain 5
• Synonyms
  • LIM
  • Enh
• GenBank acession
  • AF061258
• Locus
  4q22.3
• Discovery year
  2005-01-12
• Entrez gene record
  10611
• Pubmed identfication
  • 15346770
• Classification
  • PDZ domain containing
  • LIM domain containing
• VEGA ID
  OTTHUMG00000130973

Gene info

• Identity
  HGNC:6593
• Gene
  LHX1
• Long gene name
  LIM homeobox 1
• Synonyms
  • LIM-1
  • LIM1
• GenBank acession
  • U14755
• Locus
  17q12
• Discovery year
  1996-10-02
• Entrez gene record
  3975
• Pubmed identfication
  • 9212161
• RefSeq identity
  • NM_005568
• Classification
  • LIM class homeoboxes
• VEGA ID
  OTTHUMG00000188456

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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