HSC70 ELISA Kit

  • Catalog number
    SKT-106-96
  • Price
    Please ask
  • Size
    96 well
  • Stock availability
    In Stock
  • Kit s description
    Colorimetric detection of HSC70
  • Protein target
    HSC70
  • Scientific context
    HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
  • Primary research area
    Cancer, Heat Shock
  • Category
    Assay ELISA Kits
  • Other name
    HSC54 ELISA Kit, HSC71 ELISA Kit, HSC73 ELISA Kit, HSP71 ELISA Kit, HSP73 ELISA Kit, HSPA10 ELISA Kit, HSPA8 ELISA Kit, LAP1 ELISA Kit, NIP71 ELISA Kit
  • Brand name
    StressXpress®
  • Detection system
    Colorimetric Assay
  • Assay format
    Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
  • Main intended usage
    ELISA kit used to quantitate HSC70 concentration in samples.
  • Brief protocol
    1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
  • Kit contents
    Anti-Hsc70 Immunoassay Plate, 5X Hsc70 Extraction Reagent, Recombinant Hsc70 Standard, Standard and Sample Diluent, 10X Wash Buffer Concentrate, Anti-Hsc70 Biotinylated Antibody Concentrate, Anti-Hsc70 Biotinylated Antibody Diluent, Streptavidin: HRP Concentrate, Streptavidin: HRP Diluent, TMB Substrate, Stop Solution
  • Assay precision
    Contact us
  • Assay cross reactivity
    Human
  • Samples to be used with this kit
    Cell Lysates, Tissue, Serum
  • Maximum samples to be used with this kit
    40 samples in duplicate
  • Assay duration
    30 minutes
  • Kit s sensitivity
    1,54 ng/ml
  • Assay detection limit
    2,34 - 150 ng/ml
  • Storage recommendations
    4°C
  • Shipping recommendations
    Blue Ice
  • Bibliography
    1. Brown C.L., et al. (1993) J.Cell Biol., 120 (5): 1101-1112. 2. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591-601. 4. DeLuca-Flaherty, et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 6. Brown C.L., et al. (1996) J. Biol. Chem. 271(2): 833-840.
  • Release date
    1-Sep-2010
  • PubMed number
    25895348
  • Tested applications
    ELISA
  • Tested reactivity
    Human
  • Resources available upon request
    Kit Booklet, MSDS
  • Product image legend
    Typical Standard Curve for the HSC70 ELISA kit (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-106. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 2.34 - 150 ng/mL. Graph of the Typical Standard Curve for the HSC70 ELISA kit StressXpress - SKT-106
  • Warnings
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    2
  • Net weight g
    500
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Gene target
    HSC70  
  • Gene symbol
    HSPA8
  • Short name
    HSC70 ELISA Kit
  • Technique
    ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Alternative name
    HSC70 Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
  • Identity
  • Gene
  • Long gene name
    heat shock protein family A (Hsp70) member 8
  • Synonyms gene
  • Synonyms gene name
    • heat shock 70kD protein 8
    • heat shock 70kDa protein 8
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1995-05-08
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Spliceosomal P complex
    • Heat shock 70kDa proteins
    • Spliceosomal C complex
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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