GPR12 Antibody, FITC Conjugated

• Catalog number
  bs-13514R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  GPR12 Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  GPR12
• Specificity
  This is a highly specific antibody against GPR12.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Subcellular location
  Extracellular
• Source
  KLH conjugated synthetic peptide derived from human G protein coupled receptor 12
• Gene ID number
  2835
• Tested applications
  FCM, IF(IHC-P)
• Recommended dilutions
  FCM(1:20-100), IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR12 is a 334 amino acid peptide that is expressed primarily in brain, particularly in regions where neuronal differentiation takes place. GPR12 is coupled to an inhibitory G protein. It positively influences differentiation and maturation of post-mitotic neurons, and it may promote the growth of neuronal precursor cells.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  FLJ18149; FLJ97704; G protein coupled receptor 12; G-protein coupled receptor 12; GPCR12; GPCR21; GPR 12; GPR12; GPR12_HUMAN; MGC138349; MGC138351; Probable G protein coupled receptor GPR12; GPCR GPR12.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  GPR12   Conjugated  
• Gene symbol
  GPR12
• Short name
  Anti-GPR12
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-GPR12 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:4466
• Gene
  GPR12
• Long gene name
  G protein-coupled receptor 12
• Synonyms
  • GPCR21
  • PPP1R84
• Synonyms name
  • protein phosphatase 1, regulatory subunit 84
• GenBank acession
  • U18548
• Locus
  13q12.13
• Discovery year
  1995-01-24
• Entrez gene record
  2835
• Pubmed identfication
  • 8262253
  • 8530049
• Classification
  • Protein phosphatase 1 regulatory subunits
  • G protein-coupled receptors, Class A orphans
• VEGA ID
  OTTHUMG00000016620

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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