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Goat Anti-Rabbit IgG Antibody (H+L), Biotin Conjugated

  • Catalog number
    bs-0295G-Biotin
  • Product name
    Goat Anti-Rabbit IgG Antibody (H+L), Biotin Conjugated
  • Size
    0.2ml
  • Price
    121EUR
  • Supplier
    Bioss Secondary Antibodies
  • Ask
  • Category
    Conjugated Secondary Antibody
  • Conjugation
    Biotin
  • Host Organism
    Goat
  • Target antigen
    Rabbit IgG
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal antibody
  • Clone
    Polyclonal antibody
  • Concentration
    1ug/ul
  • Tested applications
    WB, ELISA, IHC-P, IHC-F
  • Recommended dilutions
    WB(1:500-2000), ELISA(1:500-2000), IHC-P(1:500-2000), IHC-F(1:500-2000)
  • Cross reactivity
    Rabbit
  • Background of the target antigen
    Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
  • Purification method
    This antibody has been purified via Protein A.
  • Storage conditions
    Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to one year.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Additional conjugation
    Biotinylated
  • Latin name
    Capra aegagrus hircus, Oryctolagus cuniculus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by Bioss Secondary Antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioss Secondary Antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Additional isotype
    IgG
  • French translation
    anticorps
  • Gene target
  • Short name
    Goat Anti-Rabbit IgG Antibody (H+L) Conjugated
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, goats, IgGs
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
  • Isotype
    IgG
  • Label
    Biotin
  • Species
    Goats
  • Alternative name
    caprine antibody to-production species: rabbit Immunoglobulin G (Antibody to) (heavy and light chain), biotinilated coupled
  • Alternative technique
    antibodies
  • Ask
MESH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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