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Goat anti Human lactoferrin

  • Catalog number
    GAHu/Lfr/7S
  • Product name
    Goat anti Human lactoferrin
  • Size
    10 mg
  • Price
    284.57
  • Supplier
    nordc
  • Category
    Primary Antibodies
  • Long description
    In immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma. In enzyme-immunocytochemical and immunohistochemical techniques for the indirect detection of human lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates; as catching reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). The optimum working dilution of the product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:500.
  • Antibody come from
    Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from human milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Antigen-antibody binding interaction
    Goat anti Human lactoferrin Antibody
  • Antibody is raised in
    Goat
  • Antibody's reacts with
    Human
  • Antibody's reacts with these species
    The antiSerum does not cross-react with any other Human plasma proteins as tested in gel- diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus Monkey milk has been observed.
  • Antibody's specificity
    Purified IgG fraction of polyclonal Goat antiSerum to Human lactoferrin
  • Research interest
    Cardiovascular
  • Application
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
  • Antibody's suited for
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Storage
    Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    P02788
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Latin name
    Capra aegagrus hircus
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Gene target
  • Short name
    Goat anti lactoferrin
  • Technique
    anti human, antibody to, anti human antibodies, goats
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Species
    Human, Goats, Humans
  • Alternative name
    caprine antibody to H. sapiens lactoferrin
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
Additional information
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