Goat anti Human IgG HRP

• Catalog number
  43R-1189
• Price
  Please ask
• Size
  1 mg

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Purified Human IgG, Fc fragment
• Raised in
  Goat
• Specificity
  This antibody reacts with heavy chains on human IgG (gamma chain).
• Cross Reactivity
  No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins or human IgA + IgM.
• Conjugate Tag
  HRP
• Grade Purity
  Grater than 95% pure
• Method of Purification
  Affinity purified using solid phase Human IgG (H&L)
• Source
  Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
• Concentration
  1.0 mg/ml (lot specific)
• Form Buffer
  Lyophilized powder containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.1% (v/v) Kathon CG. Protease/IgG free, pH 7.2. Rehydrate with 1.1 ml of deionized water. Prepare fresh working dilution daily.
• Storage
  Store lyophilized material at 2-8 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; WB; IHC
• Usage Recommendations
  1:200-1:5000 ELISA/WB, 1:500-1:5000 IHC
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Latin name
  Capra aegagrus hircus
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  HRP Conjugated
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Short name
  Goat anti IgG
• Technique
  anti human, anti, Goat, IgG, antibody to, anti human antibodies, goats, IgGs
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgG, IgG
• Label
  HRP
• Species
  Human, Goats, Humans
• Alternative name
  Goat anti Human IgG secondary antibody (HRP)
• Alternative technique
  antibodies, caprine, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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