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Target antigen
Gli1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
mouse, rat Theoretical reactivity:human
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Analyses
WB,IHC-P
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Immunogen
E.coli-derived human GLi1 recombinant protein (Position: Q768-A1106). Human GLi1 shares 79% amino acid (aa) sequence identity with mouse GLi1.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Gli1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Gli1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
GLI1, also known as GLI, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. It is mapped to 12q13.3. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to direct a coherent programme of neurogenesis.
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Related articles
1. Kinzler KW,; Bigner SH; Bigner DD; Trent JM; Law ML; O'Brien SJ; Wong AJ; Vogelstein B. (April 1987). "Identification of an amplified, highly expressed gene in a human glioma". Science 236 (4797): 70–3. 2. Ruiz i Altaba A. (June 1999). "Gli proteins encode context-dependent positive and negative functions: implications for development and disease.". Development 126 (14): 3205–16. 3. Sasaki H,; Hui C; Nakafuku M; Kondoh H. (April 1997). "A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro". Development 124 (7): 1313–22.
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Gene Name
GLI1
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Protein Name
Zinc finger protein GLI1
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Gene Full Name
GLI family zinc finger 1
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Synonyms
Gli 1 antibody|GLI antibody|GLI family zinc finger 1 antibody|GLI Kruppel family member 1 antibody|gli1 antibody|GLI1_HUMAN antibody|Glioma associated oncogene 1 antibody|Glioma associated oncogene antibody|Glioma associated oncogene homolog 1 (zinc finger protein) antibody|Glioma associated oncogene homolog antibody|Glioma-associated oncogene antibody|Oncogene GLI antibody|Zfp 5 antibody|Zfp5 antibody|Zinc finger protein GLI 1 antibody|Zinc finger protein GLI1 antibody
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Uniprot ID
P08151
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Entrez GeneID
2735
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps