EZLabel™ Antibody Biotin Labeling Kit

• Catalog number
  K834-5
• Price
  Please ask
• Size
  For 5 x 1 mg of antibody

• Description
  Easy to use Kit to label upto 5 mg of Antibody with Biotin for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis
• Summary
  Easy to use Kit to label upto 5 mg of Antibody with Biotin for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis
• Detection Method
  280 nm (A280).
• Species Reactivity
  N/A
• Applications
  Labeled antibodies can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis
• Sample Type
  Antibody
• Features Benefits
  All necessary reagents included. Fast and efficient labeling of antibodies with Biotin
• Storage Conditions
  +4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  BioVision’s EZLabel™ Antibody-Biotin Labeling Kit provides an easy way to label antibodies with Biotin in a user-friendly spin column format. Due to its high affinity for tetrameric avidin proteins, biotin is ideal for labeling antibodies. Each pair of spin columns provided in the kit can be used to purify up to 1 mg of the labeled target antibody. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of antibody per reaction. The biotin-labeled antibody can be directly used for multiple downstream applications including ELISA, western blot, Immunohistochemistry, Immunoassays, etc.
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  EZLabel™   Labeling   Kit  
• Short name
  EZLabel™ Antibody Labeling Kit
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Label
  Biotin
• Alternative name
  EZLabel™ (antibody to-) biotinilated Labeling reagent
• Alternative technique
  antibodies, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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