EZLabel™ Antibody FITC Labeling Kit
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Catalog number
K831-5
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Price
Please ask
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Size
For 5 x 1 mg of antibody
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Description
Easy to use Kit to label upto 5 mg of Antibody with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis
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Summary
Easy to use Kit to label upto 5 mg of Antibody with FITC for multiple applications such as ELISA, western blot, Immunohistochemistry, Immunofluorescence and FACS analysis
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Detection Method
Fluorescence (Ex/Em = 494/520 nm)
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Species Reactivity
N/A
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Applications
Labeled antibodies can be used for ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis
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Sample Type
Antibody
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Features Benefits
All necessary reagents included. Fast and efficient labeling of antibodies with FITC
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Storage Conditions
+4°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
BioVision’s EZLabel Antibody-FITC Labeling Kit provides an easy way to label antibodies with Fluorescein Isothiocyanate (FITC) in a user-friendly spin column format. FITC is an ideal dye for fluorescent labeling of antibodies among the most commonly used fluorescent dyes for labeling antibodies. Each spin column provided in the kit can be used to label up to 1 mg of the target antibody. The kit provides all of the reagents necessary to perform five labeling reactions using up to 1 mg of antibody per reaction. FITC-labeled antibody has an excitation and emission wavelengths at 494 nm and 520 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS analysis, etc.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
EZLabel™ Antibody FITC Labeling Kit
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
EZLabel™ (antibody to-) fluorecein Labeling reagent
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Alternative technique
antibodies, fluorescine, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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