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Target antigen
Estrogen Receptor beta
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
Human. No cross reactivity with other proteins.
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Estrogen Receptor beta(27-50aa HGSIYIPSSYVDSHHEYPAMTFYS), different from the related rat and mouse sequences by four amino acids.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Estrogen Receptor beta Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Estrogen Receptor beta Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Estrogen receptor-beta, referred to as ESR2, is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. This gene is mapped to 14q and comprises 8 exons spanning approximately 40 kb. ESR2 is expressed in multiple tissues, including developing spermatids of the testis and in ovarian granulosa cells. ESR-beta is homologous to the previously identified ESR-alpha and has an overlapping but nonidentical tissue distribution. The DNA-binding domain of ESR-beta is 96% conserved compared to ESR, and the ligand-binding domain shows 58% conserved residues. ESR-beta is expressed in human thymus, spleen, ovary, and testis. Rat ESR-beta is expressed in rat prostate and ovary and is homologous to rat ESR(95% conserved DNA-binding domain; 55% conserved ligand-binding domain). ESR2 mRNA was coexpressed with ESR1 and its splice variants in 60% of prolactinomas, 100% of mixed growth hormone /prolactin tumors, and 29% of gonadotroph tumors. ESR2 gene expression was not limited to ESR1-positive tumor subtypes, however, and was also found in 100% of null cell tumors, 80% of somatotroph tumors, and 60% of corticotroph tumors.
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Related articles
1. Enmark, E.; Pelto-Huikko, M.; Grandien, K.; Lagercrantz, S.; Lagercrantz, J.; Fried, G.; Nordenskjold, M.; Gustafsson, J.-A. : Human estrogen receptor beta-gene structure, chromosomal localization, and expression pattern. J. Clin. Endocr. Metab. 82: 4258-4265, 1997. 2. Mosselman, S.; Polman, J.; Dijkema, R. : ER-beta: identification and characterization of a novel human estrogen receptor. FEBS Lett. 392: 49-53, 1996. 3. Kuiper, G. G. J. M.; Enmark, E.; Pelto-Huikko, M.; Nilsson, S.; Gustafsson, J.-A. : Cloning of a novel estrogen receptor expressed in rat prostate and ovary. Proc. Nat. Acad. Sci. 93: 5925-5930, 1996. 4. Chaidarun, S. S.; Swearingen, B.; Alexander, J. M. : Differential expression of estrogen receptor-beta (ER-beta) in human pituitary tumors: functional interactions with ER-alpha and a tumor-specific splice variant. J. Clin. Endocr. Metab. 83: 3308-3315, 1998.
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Gene Name
ESR2
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Protein Name
Estrogen receptor beta(ER-beta)
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Gene Full Name
estrogen receptor 2(ER beta)
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Synonyms
ER BETA antibody|ER-beta antibody|Erb antibody|ESR 2 antibody|ESR B antibody|ESR BETA antibody|ESR2 antibody|ESR2_HUMAN antibody|ESRB antibody|ESTRB antibody|estrogen nuclear receptor beta variant a antibody|estrogen nuclear receptor beta variant b antibody|estrogen receptor 2(ER beta) antibody|Estrogen Receptor 2 antibody|estrogen receptor beta 4 antibody|Estrogen receptor beta antibody|NR3A2 antibody|Nuclear receptor subfamily 3 group A member 2 antibody
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Uniprot ID
Q92731
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translation
anticorps
