ERF/Ets2 Repressor Factor Antibody, FITC Conjugated

• Catalog number
  bs-13096R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  ERF/Ets2 Repressor Factor Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  ERF/Ets2 Repressor Factor
• Specificity
  This is a highly specific antibody against ERF/Ets2 Repressor Factor.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  KLH conjugated synthetic peptide derived from human ERF/Ets2 Repressor Factor
• Gene ID number
  2077
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  ABT1 (activator of basal transcription 1) is a nuclear protein that associates with the TATA-binding protein (TBP) and enhances basal transcription activity of class II promoters. ABT1 associates with TBP in HeLa nuclear extracts in vitro. Another protein, designated ERF, is a member of the Ets family of transcription factors. The members of the Ets family are grouped because they share a highly conserved DNA binding domain. These factors are involved in growth factor pathways and regulate both proliferation and differentiation. ERF (Ets-2 repressor factor) is a ubiquitously expressed Ets-domain protein that exhibits strong transcriptional repressor activity, suppresses Ets-induced transformation and is regulated by MAPK phosphorylation. ERF transcription may be regulated by Ets-domain proteins. Additionally, modulation of ERF activity is involved in the transcriptional regulation of genes activated during entry into G1 phase.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  ERF; ERF_HUMAN; ETS domain containing transcription factor ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE 2; PE-2; PE2.
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Description
  Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  ERF/Ets2   Repressor   Factor   Conjugated  
• Gene symbol
  ERF, ETF1
• Short name
  Anti-ERF/Ets2 Repressor Factor
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-ERF/Ets2 Repressor Factor PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:3444
• Gene
  ERF
• Long gene name
  ETS2 repressor factor
• Synonyms gene name
  • Ets2 repressor factor
• Synonyms
  • PE-2
  • PE2
• GenBank acession
  • U58535
• Locus
  19q13.2
• Discovery year
  1998-07-17
• Entrez gene record
  2077
• Pubmed identfication
  • 7588608
  • 9192842
• RefSeq identity
  • NM_006494
• Classification
  • ETS transcription factor family
• VEGA ID
  OTTHUMG00000182788

Gene info

• Identity
  HGNC:3477
• Gene
  ETF1
• Long gene name
  eukaryotic translation termination factor 1
• Synonyms gene
  • SUP45L1
  • ERF1
  • ERF
• Synonyms
  • eRF1
  • TB3-1
  • RF1
• Synonyms name
  • sup45 (yeast omnipotent suppressor 45) homolog-like 1
  • polypeptide chain release factor 1
• GenBank acession
  • AF095901
• Locus
  5q31.2
• Discovery year
  1991-06-13
• Entrez gene record
  2107
• Pubmed identfication
  • 1546371
  • 7990965
• RefSeq identity
  • NM_004730
• Classification
  • SURF complex
• VEGA ID
  OTTHUMG00000129199

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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