Type of the antibody
IgG1 monoclonal antibody
Reacts with species:
Human milk fat globule membranes.
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
Keep the Episialin, EMA Antibody ( GP1.4)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
The Episialin, EMA Antibody ( GP1.4) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1(EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type I transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
1. Lu, W.; Hisatsune, A.; Koga, T.; Kato, K.; Kuwahara, I.; Lillehoj, E. P.; Chen, W.; Cross, A. S.; Gendler, S. J.; Gewirtz, A. T.; Kim, K. C. : Cutting edge: enhanced pulmonary clearance of Pseudomonas aeruginosa by Muc1 knockout mice. J. Immun. 176: 3890-3894, 2006. 2. Sood, R.; Zehnder, J. L.; Druzin, M. L.; Brown, P. O. : Gene expression patterns in human placenta. Proc. Nat. Acad. Sci. 103: 5478-5483, 2006. 3. Wei, X.; Xu, H.; Kufe, D. : MUC1 oncoprotein stabilizes and activates estrogen receptor alpha. Molec. Cell 21: 295-305, 2006.
Gene Full Name
mucin 1, cell surface associated
CD227|EMA|PEM|PEMT|KL-6|PUM|MUCIN 1, URINARY|Episialin|H23AG|PEANUT-REACTIVE URINARY MUCIN|MUCIN, TUMOR-ASSOCIATED EPITHELIAL|POLYMORPHIC EPITHELIAL MUCIN|EPITHELIAL MEMBRANE ANTIGEN|mucin 1, transmembrane|Breast carcinoma-associated antigen DF3|Carcinoma-associated mucin|Krebs von den Lungen-6|Peanut-reactive urinary mucin|Polymorphic epithelial mucin|Tumor-associated epithelial membrane antigen|Tumor-associated mucin
Human(15941), Mouse(02496), Rat(B2GV31)
Human(4582), Mouse(17829), Rat(24571)
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.