Dnmt3a Antibody
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Catalog numberF40603-0.08ML
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PricePlease ask
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Size0.08 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenDnmt3a
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC, ELISA
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Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:50-1:100
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NotesTitration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis Dnmt3a antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ9Y6K1
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PurityPurified
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DescriptionDnmt3a is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. [UniProt]
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ImmunogenA portion of amino acids 457-486 from the human protein was used as the immunogen for this Dnmt3a antibody.
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StorageAliquot the Dnmt3a antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolDNMT3A, LINC01381
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Short nameAnti-Dnmt3a
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to Dnmt3a
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameDNA methyltransferase 3 alpha
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Synonyms gene name
- DNA (cytosine-5-)-methyltransferase 3 alpha
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- 7BS C5-cytosine DNA/RNA methyltransferases
- PWWP domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 1381
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2014-06-04
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Entrez gene record
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data