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Target antigen
Daxx
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Daxx(325-341aa QERRHLDLIYNFGCHLT), identical to the related mouse and rat sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Daxx Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Daxx Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
DAXX(death-domain associated protein) also known as DAP6(Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses by Yang et al.(1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.
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Related articles
1. Chang, H. Y., Nishitoh, H., Yang, X., Ichijo, H., Baltimore, D. Activation of apoptosis signal-regulating kinase 1 (ASK1) by the adapter protein Daxx. Science 281: 1860-1863, 1998. 2. Kiriakidou, M., Driscoll, D. A., Lopez-Guisa, J. M., Strauss, J. F., III. Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol. 16: 1289-1298, 1997. 3. Yang, X., Khosravi-Far, R., Chang, H. Y., Baltimore, D. Daxx, a novel Fas-binding protein that activates JNK and apoptosis. Cell 89: 1067-1076, 1997.
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Gene Name
DAXX
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Protein Name
Death domain-associated protein 6
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Gene Full Name
death-domain associated protein
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Synonyms
antibody|BING 2 antibody|BING2 antibody|CENP-C binding protein antibody|DAP 6 antibody|DAP6 antibody|Daxx antibody|DAXX antibody|DAXX_HUMAN antibody|Death associated protein 6 antibody|Death domain associated protein 6 antibody|Death domain associated protein antibody|Death domain-associated protein 6 antibody|EAP 1 antibody|EAP1 antibody|ETS1 associated protein 1 antibody|ETS1-associated protein 1 antibody|Fas binding protein antibody|Fas death domain associated protein antibody|Fas death domain-associated protein antibody|hDaxx antibody|MGC126245 antibody|MGC126246 antibody
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Uniprot ID
Q9UER7
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
