Acidic Cytokeratins monoclonal antibody

  • Catalog number
    11-768-C025
  • Price
    Please ask
  • Size
    25ug
  • Long name
    Mouse Monoclonal Antibody against Human Acidic Cytokeratins, Clone: AE1
  • Antigen
    Acidic Cytokeratins
  • Species reactivity
    Human (Homo sapiens), Non-Human (Homo sapiens) Primates, Mouse (Mus musculus), Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    AE1
  • Applications
    FC, WB, IHC(P), IHC(F), ICC
  • Immunogen
    Human epidermal keratins
  • Format conjugation
    purified; unconjugated
  • Specificity
    Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins, namely K10, 14, 15, 16, 19 (40-56 Kda). This antibody stains well the basal layer of epidermis and most epithelia.
  • Research area
    Cytoskeleton: Intermediate Filaments (Veterinary), Cytoskeleton: Intermediate Filaments (Rodent), Cytoskeleton: Intermediate Filaments (Human)
  • Concentration
    1 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Mouse Monoclonal Antibody against Human Acidic Cytokeratins, Clone: AE1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by protein-A affinity chromatography
  • References
    *Listrom MB, Dalton LW: Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2. Am J Clin Pathol. 1987 Sep;88(3):297-301., *Hunt RC, Davis AA: Altered expression of keratin and vimentin in human retinal pigment epithelial cells in vivo and in vitro. J Cell Physiol. 1990 Nov;145(2):187-99., *Ansai SI, Katagata Y, Yoshikawa KI, Hozumi Y, Aso K: Keratin specificity analyses of eight anti-keratin monoclonal antibodies, and their immunostaining patterns in normal skin using formalin-fixed and paraffin-embedded tissue specimens. Arch Dermatol Res. 1993;285(1-2):6-12., *Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8.
  • Properties
    If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti-Acidic Cytokeratins Mab
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Host
    Mouse (Mus musculus)
  • Isotype
    IgG1
  • Alternative name
    Mouse Monoclonal to Acidic Cytokeratins
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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