anti- Cleaved LC3 antibody

• Catalog number
  FNab04716
• Price
  Please ask
• Size
  2X100μg

• Form
  liquid
• Purification
  Immunogen affinity purified
• Purity
  ≥95% as determined by SDS-PAGE
• Storage
  PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
• Background
  MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
• Immunogen
  microtubule-associated protein 1 light chain 3 alpha
• Synonyms
  LC3, LC3A, LC3A/B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A
• Calculated MW
  Refer to Figures
• Uniprot ID
  Q9H492
• Specificity
  Mouse, Rat
• Tested Application
  ELISA, WB, IHC
• Recommended dilution
  WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Image1
  Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab04716 (LC3 Antibody) at dilution of 1:1000
• Image4
  Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab04716( LC3 antibody) at dilution of 1:1000
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Cleaved   LC3  
• Gene symbol
  MAP1LC3A
• Short name
  anti- Cleaved LC3 antibody
• Technique
  Antibody, anti, antibody to, antibodies against human proteins, antibodies for
• Host
  Rabbit
• Isotype
  IgG
• Alternative name
  antibody to- Cleaved LC3 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6838
• Gene
  MAP1LC3A
• Long gene name
  microtubule associated protein 1 light chain 3 alpha
• Synonyms
  • MAP1BLC3
  • MAP1ALC3
  • LC3
  • LC3A
  • ATG8E
• Locus
  20q11.22
• Discovery year
  2000-06-29
• Entrez gene record
  84557
• Pubmed identfication
  • 8833088
  • 17580304
• RefSeq identity
  • NM_181509
• Classification
  • Autophagy related
• VEGA ID
  OTTHUMG00000032306

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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