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Also known as
CEACAM 1,5,6,8
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Other names
carcinoembryonic antigen-related cell adhesion molecule 5 preproprotein; Carcinoembryonic antigen-related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100; carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen; CEA; Meconium antigen 100
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CEACAM5; CEACAM5; CEA; CD66e; CEA; CEA
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Gene name synonims
CEACAM5; CEACAM5; CEA; CD66e; CEA; CEA
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Other gene names
CEACAM5; CEACAM5; CEA; CD66e; CEA; CEA
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2b
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Clone
5E503
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Broadly reactive antibody which reacts with most of the CEACAM molecules characterized to date (except CEACAM3, CEACAM4 and CEACAM7) (4). Can be used to detect CEACAM1 (BGP/CD66a), CEACAM5 (CEA/CD66e), CEACAM6 (NCA/CD66c) and CEACAM8 (CGM6/CD66b) on transiently transfected BOSC23 cells by flow cytometry. Included and characterized in the studies from the VIth Leucocyte Typing Workshop (4).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC23 cells transiently transfected with a CEACAM5 expression vector.
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Form Appearance
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze the antibody should be stored at -20 degrees Celsius.. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the CEACAM 1 5 6 8 (Carcinoembryonic Antigen Adhesion Molecule) Antibody. Antigens are peptides or recombinant or native dependent on the production method. For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps