CDK1 (Tyr15) Antibody, Cy3 Conjugated

• Catalog number
  bs-3092R-Cy3
• Price
  Please ask
• Size
  0.1ml

• Long name
  CDK1 (Tyr15) Polyclonal Antibody, Cy3 Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Cy3
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  CDK1 Tyr15
• Specificity
  This is a highly specific antibody against CDK1 Tyr15. This phosphorylation site is homologous in the listed cross reactive species at the specified location.
• Modification
  Phosphorylation
• Modification site
  Tyr15
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  5-25/297
• Subcellular location
  Cytoplasm, Nucleus, Mitochondrion
• Source
  KLH conjugated synthetic phosphopeptide derived from human CDK1 around the phosphorylation site of Tyr15 [GT(p-Y)GV]
• Gene ID number
  983
• Swiss Prot
  P06493
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  512,550nm/570,615nm
• Synonyms
  CDC2; CDC28A; P34CDC2; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDKN1
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-CDK1 Tyr15 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
• Additional conjugation
  cy3 conjugation kit
• French translation
  anticorps

• Gene target
  CDK1   Tyr15   Conjugated  
• Gene symbol
  CDK1
• Short name
  Anti-CDK1 Tyr15
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Cy3
• Alternative name
  Anti-CDK1 Tyr15 PAb Cy3
• Alternative technique
  antibodies
• Alternative to gene target
  cyclin-dependent kinase 1, CDC2 and CDC28A and P34CDC2, CDK1 and IDBG-75344 and ENSG00000170312 and 983, transferase activity, nuclei, Cdk1 and IDBG-160472 and ENSMUSG00000019942 and 12534, CDK1 and IDBG-628960 and ENSBTAG00000010109 and 281061

Gene info

• Identity
  HGNC:1722
• Gene
  CDK1
• Long gene name
  cyclin dependent kinase 1
• Synonyms gene
  • CDC2
• Synonyms gene name
  • cell division cycle 2, G1 to S and G2 to M
• Synonyms
  • CDC28A
• GenBank acession
  • BC014563
• Locus
  10q21.2
• Discovery year
  1986-01-01
• Entrez gene record
  983
• Pubmed identfication
  • 3553962
  • 19884882
• RefSeq identity
  • NM_001786
• Classification
  • Cyclin dependent kinases
• VEGA ID
  OTTHUMG00000018290

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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