CDK1 Antibody, FITC Conjugated

• Catalog number
  bs-0542R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  CDK1 Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  CDK1
• Specificity
  This is a highly specific antibody against CDK1.
• Modification
  Unmodified
• Modification site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  250-297/297
• Subcellular location
  Cytoplasm, Nucleus, Mitochondrion
• Source
  KLH conjugated synthetic peptide derived from mouse CDK1
• Gene ID number
  12534
• Swiss Prot
  P11440
• Tested applications
  FCM, IF(IHC-P)
• Recommended dilutions
  FCM(1:20-100), IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  Cdc2; Cdc2a; p34; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; Cdkn1
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  CDK1   Conjugated  
• Gene symbol
  CDK1
• Short name
  Anti-CDK1
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-CDK1 PAb FITC
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  cyclin-dependent kinase 1, CDC2 and CDC28A and P34CDC2, CDK1 and IDBG-75344 and ENSG00000170312 and 983, transferase activity, nuclei, Cdk1 and IDBG-160472 and ENSMUSG00000019942 and 12534, CDK1 and IDBG-628960 and ENSBTAG00000010109 and 281061

Gene info

• Identity
  HGNC:1722
• Gene
  CDK1
• Long gene name
  cyclin dependent kinase 1
• Synonyms gene
  • CDC2
• Synonyms gene name
  • cell division cycle 2, G1 to S and G2 to M
• Synonyms
  • CDC28A
• GenBank acession
  • BC014563
• Locus
  10q21.2
• Discovery year
  1986-01-01
• Entrez gene record
  983
• Pubmed identfication
  • 3553962
  • 19884882
• RefSeq identity
  • NM_001786
• Classification
  • Cyclin dependent kinases
• VEGA ID
  OTTHUMG00000018290

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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