CD154 / CD40L monoclonal antibody

  • Catalog number
    1F-629-C100
  • Price
    Please ask
  • Size
    100ug
  • Long name
    Hamster Monoclonal Antibody against Mouse CD154 / CD40L, Clone: MR-1
  • Antigen
    CD154 / CD40L (mouse)
  • Species reactivity
    Mouse (Mus musculus)
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Monoclonal Antibody (Mab)
  • Clone
    MR-1
  • Applications
    FC, IP, IHC, ICC, FUNC
  • Immunogen
    Activated mouse Th1 clone D1.6_x000D_
  • Format conjugation
    FITC
  • Specificity
    The Armenian hamster monoclonal antibody MR-1 detects mouse CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes. _x000D_
  • Research area
    CD and Related Antigens & MHC (Rodent)
  • Concentration
    0.5 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Hamster Monoclonal Antibody against Mouse CD154 / CD40L, Clone: MR-1 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center._x000D_
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified by affinity chromatography
  • References
    _x000D_ , *Lode HN, Xiang R, Pertl U, Förster E, Schoenberger SP, Gillies SD, Reisfeld RA: Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction. J Clin Invest. 2000 Jun;105(11):1623-30._x000D_ , *Fischbein MP, Ardehali A, Yun J, Schoenberger S, Laks H, Irie Y, Dempsey P, Cheng G, Fishbein MC, Bonavida B: CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants. J Immunol. 2000 Dec 15;165(12):7316-22._x000D_ , *Hwang Y, Nahm MH, Briles DE, Thomas D, Purkerson JM: Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect Immun. 2000 Feb;68(2):511-7._x000D_ , *Murakawa T, Kerklo MM, Zamora MR, Wei Y, Gill RG, Henson PM, Grover FL, Nicolls MR: Simultaneous LFA-1 and CD40 ligand antagonism prevents airway remodeling in orthotopic airway transplantation: implications for the role of respiratory epithelium as a modulator of fibrosis. J Immunol. 2005 Apr 1;174(7):3869-79._x000D_ , *Salgado CG, Nakamura K, Sugaya M, Tada Y, Asahina A, Koyama Y, Irie S, Tamaki K: Functional CD40 ligand is expressed on epidermal Langerhans cells. J Leukoc Biol. 1999 Aug;66(2):281-5._x000D_ , *Wu Y, Zhou Q, Zheng P, Liu Y: CD28-independent induction of T helper cells and immunoglobulin class switches requires costimulation by the heat-stable antigen. J Exp Med. 1998 Apr 6;187(7):1151-6._x000D_ , *Luzina IG, Atamas SP, Storrer CE, daSilva LC, Kelsoe G, Papadimitriou JC, Handwerger BS: Spontaneous formation of germinal centers in autoimmune mice. J Leukoc Biol. 2001 Oct;70(4):578-84. _x000D_ , *Shin T, Kennedy G, Gorski K, Tsuchiya H, Koseki H, Azuma M, Yagita H, Chen L, Powell J, Pardoll D, Housseau F: Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor. J Exp Med. 2003 Jul 7;198(1):31-8._x000D_ , *Howard LM, Miga AJ, Vanderlugt CL, Dal Canto MC, Laman JD, Noelle RJ, Miller SD: Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis. J Clin Invest. 1999 Jan;103(2):281-90._x000D_ , *Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A: A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6550-4._x000D_
  • Properties
    If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    CD40LG
  • Short name
    Anti-CD154 / CD40L Mab
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
  • Isotype
    IgG1
  • Alternative name
    Hamster Monoclonal to CD154 / CD40L (mouse)
  • Alternative technique
    antibodies
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