B And T-Lymphocyte Attenuator (BTLA) Polyclonal Antibody (Rat), FITC
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Catalog number
PAB723Ra01-10ml-FITC
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Price
Please ask
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Size
10ml
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Description
A Rabbit polyclonal antibody against Rat B And T-Lymphocyte Attenuator (BTLA). This antibody is labeled with FITC.
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Specifications
Host: Rabbit; Species Reactivity: Rat; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: FITC
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Additional_information
Sequence of the immunogen: BTLA (Lys30~Tyr183); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
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Notes
Research Use Only.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
BTLA
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Short name
B T-Lymphocyte Attenuator (BTLA) Polyclonal Antibody ( ), FITC
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Technique
Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rat
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Label
FITC
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Species
Rat, Rats
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Alternative name
B And T-Lymphocyte Attenuator (B and T lymphocyte associated) polyclonal (antibody to-) (Rat), fluorecein
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Alternative technique
polyclonals, antibodies, fluorescine
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Alternative to gene target
B and T lymphocyte associated, BTLA and IDBG-49858 and ENSG00000186265 and 151888, protein binding, Cell surfaces, Btla and IDBG-162900 and ENSMUSG00000052013 and 208154, BTLA and IDBG-632288 and ENSBTAG00000011871 and 531767
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Tissue
lymphocyte
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Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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