ATG12 Antibody

• Catalog number
  F41508-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  ATG12
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IF, ELISA
• Recommended dilutions
  Western blot: 1:1000,Immunofluorescence: 1:50-1:100
• Notes
  Titration of the ATG12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This ATG12 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O94817
• Purity
  Antigen affinity
• Description
  Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).APG12L/ATG12 is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.
• Immunogen
  A portion of amino acids 1-50 from the human protein was used as the immunogen for this ATG12 antibody.
• Storage
  Aliquot the ATG12 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  ATG12  
• Gene symbol
  ATG12
• Short name
  Anti-ATG12
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to ATG12
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:588
• Gene
  ATG12
• Long gene name
  autophagy related 12
• Synonyms gene
  • APG12L
• Synonyms gene name
  • Apg12 (autophagy 12, S. cerevisiae)-like
  • APG12 autophagy 12-like (S. cerevisiae)
  • ATG12 autophagy related 12 homolog (S. cerevisiae)
• Synonyms
  • APG12
• Synonyms name
  • APG12 autophagy 12-like
• GenBank acession
  • AB017507
• Locus
  5q22.3
• Discovery year
  1999-10-01
• Entrez gene record
  9140
• Pubmed identfication
  • 9852036
• RefSeq identity
  • NM_004707
• Classification
  • Autophagy related
• VEGA ID
  OTTHUMG00000128889

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products