antibody to or anti- biotin conjugated -FITC conjugated antibody
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Catalog number
GWB-987F63
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Price
Please ask
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Size
1 vial
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Old catalog number
18-251-404368
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Storage temperature
store cold
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Expiry date
1 y
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Category
research antibodies, ELISAs, recombinant proteins, vectors and reagents for laboratory use
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by genways they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This genways Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. genways FITC is highly sensitive to pH extremes.
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Conjugation
Biotinylated, Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
antibody anti- conjugated -FITC conjugated antibody
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Technique
Antibody, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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Label
Biotin
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Alternative name
(antibody to-) to and antibody to- biotinilated coupled -fluorecein coupled (antibody to-)
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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