This antibody reacts with:
Human, mouse, rat
Synthetic peptide mapping to the C-terminus of human Wee1
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity-purified rabbit anti-Wee1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Alternative names of antibody target:
WEE1hu, Wee1A kinase, DKFZp686I18166, FLJ16446, EC 184.108.40.206
Western blot analysis (0.5-4 µg/ml) and in in immunoprecipitation (5-10 µg/ml). However, the optimal conditions should be determined individually. The affinity-purified rabbit antibody recognizes Wee1 protein from human, mouse and rat samples. Mouse small intestine tissue lysate (Cat.# 2601-100) can be used as a positive control
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Wee1 is a nuclear tyrosine protein kinase. The protein plays a role in suppressing eukaryotes’ entry into mitosis by inactivating cdc2 kinase by inhibiting tyrosine phosphorylation. The predicted molecular weight of the kinase is 72 kDa. The kinase possesses tyrosine kinase activity, but structurally belongs to the serine/threonine protein kinase family.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.