This antibody reacts with:
Human, mouse, rat
Fusion protein of human USP3
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Alternative names of antibody target:
USP3, MGC129878, MGC129879, SIH003, UBP
Western blot: 1:500 – 1:1000 dilution. However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP3 (ubiquitin specific peptidase 3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBPtype zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin Cterminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.