Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. antibody detects 30 and 55 kDa isoforms of TGF-β Receptor I on SDS-PAGE immunoblots in samples from human, mouse, rat, pig and bovine origins.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
TGF-β (Transforming growth factor-beta superfamily members are critical regulators of cell proliferation, differentiation, morphogenesis, and pathogenesis. TGF-beta receptor is a serine/threonine kinase receptor complex that consists of two distinct transmembrane proteins known as type I and type II receptors. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and thus activation of the type I receptor kinases.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.