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anti-SETDB1 Antibody

  • Catalog number
    6155-100
  • Product name
    anti-SETDB1 Antibody
  • Size
    SETDB1 Antibody
  • Price
    414EUR
  • Supplier
    biovision antibodies-kits-proteins
  • Ask
  • Antibody host:
    Rabbit
  • This antibody reacts with:
    Human, mouse
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Fusion protein of human SETDB1
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blot, Immunohistochemistry, immunoprecipitation
  • Target of this antibody:
    SETDB1
  • Formulation of this antibody:
    100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
  • Alternative names of antibody target:
    ESET, H3­K9­HMTase4, KG1T, KIAA0067, KMT1E.
  • Recommended usage:
    Western blot: 1:500 – 1:1000, IHC: 1:50 – 1:100, IP: 1:50 – 1:100
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Polyclonal
  • Isotype:
    Rabbit IgG
  • Background information:
    The Erg­associated protein with SET domain (ESET), also known as SET domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila, Enhancer of zeste, and Trithorax proteins. ESET also contains tudor and methyl­CpG­binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively. ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing. However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters. ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co­repressors (mSin3A/B, MBD1, KAP­1, the ATFa­associated modulator mAM). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di­methyl to tri­methyl histone H3 Lys9. MBD1 recruits ESET to the CAF­1 complex to facilitate methylation of histone H3 Lys9 during replication­coupled chromatin assembly in S phase. DNMT3A recruits ESET to silenced promoters in cancer cells. ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri­methyl histone H3 Lys9 are both increased in diseased brains.
  • Concentration of antibody:
    1 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-SETDB1 Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-SETDB1 (Antibody to)
  • Alternative technique
    antibodies
  • Ask
1. Gene info
MESH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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