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anti-Red Fluorescent Protein (dsRed) Antibody

  • Catalog number
    3994-100
  • Product name
    anti-Red Fluorescent Protein (dsRed) Antibody
  • Size
    Red Fluorescent Protein (dsRed) Antibody
  • Price
    360EUR
  • Supplier
    biovision antibodies-kits-proteins
  • Ask
  • Antibody host:
    Mouse
  • This antibody reacts with:
    All species
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Recombinant Red Fluorescent Protein (dsRed) expressed from E. coli (Cat.# 4997-100)
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blot, Immunofluorescence, Immunohistochemistry
  • Target of this antibody:
    RFP (dsRed)
  • Formulation of this antibody:
    100 μg (0.5 mg/ml) Protein A affinity purified mouse anti-dsRed monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
  • Alternative names of antibody target:
    Red Fluorescent Protein
  • Recommended usage:
    The antibody can be used for Western blotting (1-4 μg/ml), immunofluorescence (10-20 μg/ml) and immunohistochemistry (10-20 μg/ml). However, the optimal conditions should be determined individually.
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Monoclonal
  • Isotype:
    Mouse IgG2b
  • Clone:
    BV3-2
  • Background information:
    Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.
  • Concentration of antibody:
    0.5 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Conjugation
    red dye
  • Gene target
  • Short name
    anti-Red Protein (dsRed) Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Label
    Red
  • Alternative name
    antibody to-Red Fluorescent Protein (dsRed) (Antibody to)
  • Alternative technique
    antibodies
  • Ask
MESH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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