Western blotting (1:200-1000) and immunofluorescence (1:50-100). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects a ~60 kDa Tyrosine Hydroxylase when phosphorylated at Ser40.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Tyrosine hydroxylase play an important role in the synthesis of dopamine and other catecholamines. The enzyme’s activity is regulated in the level of transcription and translation, as well as posttranscriptional modifications. Phosphorylation at Ser40 may play a key role in regulation of the catalytic activity of the enzyme.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.