This antibody reacts with:
Human, mouse, rat
Synthetic peptide near the C-terminus of human p70 S6 kinase
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Target of this antibody:
p70 S6 Kinase
Formulation of this antibody:
100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
Alternative names of antibody target:
RPS6KB1 , p70S6k , p70 , S6K , p70(S6K)-alpha , P70S6K , p70(S6K)-alpha , p70-alpha
Western blotting (1 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes p70 S6 kinase of human, mouse, and rat origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
p70 S6 kinase and p90 Rsk kinase are the two distinct 40S ribosomal protein S6 Ser/Thr kinases present in somatic animal cells. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, sμggesting that specific kinases may exist upstream in the signaling pathways that regulate these kinases.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.