This antibody reacts with:
Human, mouse, rat
Synthetic peptide mapping to the N-terminus of human p53DINP1
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg(0.5 mg/ml) affinity purified rabbit anti-p53DINP1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
TP53DINP1 Teap, TP53DINP1A,p53DINP1, P53DINP1,TP53DINP1, SIP , FLJ22139, DKFZp434M1317, TP53INP1B
Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5-10 µg/ml). However, the optimal dilution conditions should be determined individually. Human Lung tissue lysate can be used as a positive control. The antibody detects the 27 kDa p53DINP1-α in human, mouse, and rat samples. A lower band (~18 kDa) which represents the p53DINP1-β was also detected in certain tissue and cells.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
p53DINP1 (p53-dependent damage-inducible nuclear protein 1) encodes two proteins termed p53DINP1-α (27 kDa) and p53DINP1-β (18 kDa). p53DINP1 may regulate p53-dependent apoptosis throμgh phosphorylation at Ser46 and induction of p53AIP1. p53DINP1 is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.