Western Blot (1 µg/ml) using secondary antibody coupled to alkaline phosphatase and BCIP/NBT as substrate, ELISA (1:1000), Immunohistochemistry.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is a marker for inflammation and nitric oxide (NO) production. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosine residues important for signal transduction. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, chronic renal failure and keratoconus. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.