This antibody reacts with:
E. coli-expressed human MMP-2 (Cat.# 7782-10)
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
30 µg (0.5 mg/ml) affinity purified rabbit anti-human MMP-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
MMP2, MMP-2, MMP 2, Matrix metalloproteinase-2, Matrix metalloproteinase 2, Gelatinase A, TBE-1
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Cross-reactivity to MMP-2 of other species has not been determined.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
The mammalian Matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids, the MMPs are secreted in latent forms. Upon activation, the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-2 (72 kDa type IV collagenase, Gelatinase-A) contains the basic structure of propeptide, catalytic, and hemopexin domains. It is an important proteinase in tissue remodeling.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.