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anti-Heme Oxygenase-2 Antibody

  • Catalog number
    3392-100
  • Product name
    anti-Heme Oxygenase-2 Antibody
  • Size
    Heme Oxygenase-2 Antibody
  • Price
    338EUR
  • Supplier
    biovision antibodies-kits-proteins
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  • Antibody host:
    Rabbit
  • This antibody reacts with:
    Human, mouse, rat, monkey, hamster
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Synthetic peptide surrounding amino acid 34 of human HO-2
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blotting, Immunoprecipitation, Immunohistochemistry
  • Target of this antibody:
    Heme Oxygenase-2
  • Formulation of this antibody:
    100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
  • Alternative names of antibody target:
    HMOX2 , HO-2 ,HO2 , EC 1.14.99.3
  • Recommended usage:
    Western blotting (0.5-4 µg/ml), in immunoprecipitation (20 µg/ml) and Immunohistochemistry (20 µg/ml, frozen section tested). However, the optimal concentrations should be determined individually. The antibody recognizes ~36 kDa Heme-Oxygenase-2 of human, mouse, rat, hamster, and monkey origins. Blocking peptide (Cat.# 3392BP-50) is available separately.
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Polyclonal
  • Isotype:
    Rabbit IgG
  • Background information:
    Heme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme, a pro-oxidant, into carbon monoxide and biliverdin. Two heme oxygenase isozymes, termed HO-1 and HO-2, have been identified so far. At the amino acid level, HO-1 and HO-2 are 42% homologous. However, each isozyme is evolutionary highly conserved. HO-1 is the inducible form of the enzyme and is therefore also referred to as stress protein Hsp32. Expression of HO-1 can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO-2 is the constituitive form of the enzyme. It is not inducible by exogeneous stimuli and HO-2 is the most prevalent form in most tissues, except the spleen where HO-1 levels are predominant under normal conditions.
  • Concentration of antibody:
    0.5 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-Heme Oxygenase-2 Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-Heme Oxygenase-2 (Antibody to)
  • Alternative technique
    antibodies
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MESH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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