Donkey anti Goat IgG H + L Alexa Fluor 647
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Catalog number43R-ID028AF
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PricePlease ask
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Size500 µg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Raised inDonkey
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SpecificityGoat IgG
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Cross ReactivityMinimal cross reaction with Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, Rat
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IsotypesIgG
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Conjugate TagAlexa Fluor 647
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Method of PurificationDonkey anti Goat IgG (H + L) (Alexa Fluor 647) was purified by affinity chromatography.
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Concentration1.4 mg/ml
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Form BufferLyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.
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StorageStore at 2-8 deg C until reconstitution. Following reconstitution aliquot and freeze at -70 deg C for long term storage. Avoid repeated freeze-thaw cycles.
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Shipping conditionsBlue Ice
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Tested forPlease refer to the datasheet
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Usage Recommendations1:100 - 1:800 for most applications.
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Additional InformationAlexa Fluor 647 Carboxylic Acid, Amax=651; Emax=667. Absorbance Ratio: A651/A280 = 4.3
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. fitzgerald made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the anti Goat IgG H + L exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.
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ConjugationAlexa Fluor, ALEXA FLUOR 647
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Latin nameEquus africanus asinus, Capra aegagrus hircus
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target-
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Gene symbolCALD1, FUT1, L3MBTL2
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Short nameanti Goat IgG H + L
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Techniqueanti, Goat, IgG, antibody to, goats, IgGs
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HostGoat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeIgG, IgG
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LabelALEXA FLUOR 647
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SpeciesDonkeys, Goats
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Alternative nameDonkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)
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Alternative techniqueantibodies, caprine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namecaldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 1 (H blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
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Synonyms name
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Locus
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Discovery year1989-06-06
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Entrez gene record
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameL3MBTL histone methyl-lysine binding protein 2
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Synonyms gene name
- l(3)mbt-like 2 (Drosophila)
- L3MBTL2, polycomb repressive complex 1 subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-04-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MBT domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data