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anti-Gai2 Antibody

  • Catalog number
    3249-100
  • Product name
    anti-Gai2 Antibody
  • Size
    Gai2 Antibody
  • Price
    371.58
  • Supplier
    biovision antibodies-kits-proteins
  • Antibody host:
    Rabbit
  • This antibody reacts with:
    Human
  • Purification method:
    Affinity purified
  • Immunogene sequence:
    Synthetic peptide surrounding amino acid 229 of human Gai2
  • How to handle this antibody:
    The antibody solution should be gently mixed before use.
  • Form of antibody:
    Liquid
  • This antibody has been succesfully tested for use in following methods:
    Western blot, Immunoprecipitation
  • Target of this antibody:
    Gai2
  • Formulation of this antibody:
    100 µg (0.5 mg/ml) affinity-purified rabbit anti-Gαi2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
  • Alternative names of antibody target:
    guanine nucleotide binding protein G, GNAI2B, guanine nucleotide binding protein
  • Recommended usage:
    Western blot (at 0.5-4 µg/ml) and immunoprecipitation. However, the optimal conditions should be determined individually.
  • Storage information:
    Shelf time: 12 months in temp.: -20 deg.C
  • Shipping conditions:
    gel pack
  • Clonality of antibody:
    Polyclonal
  • Isotype:
    Rabbit IgG
  • Background information:
    Gαi2 is a 40-41 kDa alpha-subunit of heterotrimeric protein that shares sequence homology with Gαi3 and Gαi1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence, interfering with receptor coupling. Gαi2 is expressed widely in tissues and cell lines. Activation of PLC-β by Gαi-coupled receptors is the result of release of Gβg subunits from Gαi. Gαi2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase.
  • Concentration of antibody:
    0.5 mg/ml
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Gene target
  • Short name
    anti-Gai2 Antibody
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for
  • Alternative name
    antibody to-Gai2 (Antibody to)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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