Western blotting (1-4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control and an approximately 40 kDa band should be detected by Western blotting.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). FasL is a tumor necrosis factor (TNF)-related type II membrane protein and is expressed in various tissue and cells. Both Fas and FasL are thoμght to play an important role in the apoptosis processes that takes place during T cell development.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.