This antibody reacts with:
Human, mouse, rat
Synthetic peptide near the C-terminal of mouse EDEM1
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) antibody in 1 x PBS pH 7.2, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.
Alternative names of antibody target:
ER degradation-enhancing alpha-mannosidase-like protein 1
Western blot: (1:200 dilution) Use 0.5 – 4 µg/ml based on your sample type.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
EDEM1 belongs to the glycosyl hydrolase 47 family. It extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-dependent manner.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.