This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 438 of mouse DMAP1
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
Alternative names of antibody target:
DNMAP 1 , DNMAP1 , EAF 2 , EAF2 , FLJ11543 , KIAA1425 , MGC55593 , SWC 4 , SWC4 , zgc:55593
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate (Cat.# 2401-100) and mouse small intestine tissue lysate (Cat.2601-100) can be used as positive controls. The antibody recognizes the DMAP1 of human, mouse and rat origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
DNA methylation can help regulate transcriptional silencing via repressive transcription complexes, which include methyl-CpG-binding domain protein and Histone deacetylases (HDACs) DNMT1. This core enzyme for mammalian DNA methylation can also establish a repressive transcription complex consisting of DNMT1, HDAC2 and DMAP1. DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1 exists throμghout the S-phase; HDAC2 joind DNMT1 and DMAP1 only during Late S phase. This provides a regulated means to deacetylate heterochromatin following replication
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.