anti-DFF45 Antibody
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Catalog number1148
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PricePlease ask
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Size0.02 mg, 0.1 mg
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Gene infoDFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
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Gene namesDFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
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Antibodie s labelunlabelled serum
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PurityDFF45 Antibody is Antibody is DEAE purified.
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Ab raised inRabbit
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TypePolyclonal
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ImmunoglobulinIgG
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AntigenDFF45 antibody was made against a 20 amino acid peptide near the amino terminus of human DFF45.
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Immuno applicationELISA, Western Blot
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Antibody adviceDFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells.
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Suspention pH azideDFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
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Keep atDFF45 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Works withHuman
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Omim nr4758148
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Ncbi nrNP_004392
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KDa45 and 35 kDa
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Vial with antibodyin solution
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NCBI GENE nr1676
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GENE symbolDFFA
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Antigene nameDNA fragmentation factor, 45kDa, alpha polypeptide
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SourceHomo sapiens
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Protein nrO00273
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolDFFA
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Short nameanti-DFF45 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toDFF45 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameDNA fragmentation factor subunit alpha
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Synonyms gene name
- DNA fragmentation factor, 45 kD, alpha polypeptide
- DNA fragmentation factor, 45kDa, alpha polypeptide
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data