anti-DFF45 Antibody

  • Catalog number
    1148
  • Price
    Please ask
  • Size
    0.02 mg, 0.1 mg
  • Gene info
    DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
  • Gene names
    DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
  • Antibodie s label
    unlabelled serum
  • Purity
    DFF45 Antibody is Antibody is DEAE purified.
  • Ab raised in
    Rabbit
  • Type
    Polyclonal
  • Immunoglobulin
    IgG
  • Antigen
    DFF45 antibody was made against a 20 amino acid peptide near the amino terminus of human DFF45.
  • Immuno application
    ELISA, Western Blot
  • Antibody advice
    DFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells.
  • Suspention pH azide
    DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
  • Keep at
    DFF45 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Works with
    Human
  • Omim nr
    4758148
  • Ncbi nr
    NP_004392
  • KDa
    45 and 35 kDa
  • Vial with antibody
    in solution
  • NCBI GENE nr
    1676
  • GENE symbol
    DFFA
  • Antigene name
    DNA fragmentation factor, 45kDa, alpha polypeptide
  • Source
    Homo sapiens
  • Protein nr
    O00273
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    DFF45  
  • Gene symbol
    DFFA
  • Short name
    anti-DFF45 Antibody
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
  • Alternative name
    Antibody toDFF45 (antibody to-)
  • Alternative technique
    antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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