This antibody reacts with:
Human, mouse, rat, rabbit, pig
Synthetic peptide surrounding amino acid 130 of human Decorin
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Decorin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 25% glycerol, 0.5% BSA, and 0.01% thimerosal.
Alternative names of antibody target:
DCN , 125255 , P07585 , DSPG2 , PG40 , PGII , PGS2 , SLRR1B
Western blotting (0.5-4 µg/ml), and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects ~22 kDa protein representing the Decorin fragment generated by digestion of human Decorin by MMP-3.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Decorin is a member of the small, leucine-rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF-β. It is believed that decorin also plays a role in the cell cycle of chondrocytes.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.