Western blotting (0.5-4 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. Recognizes human cystatin B. Does not cross-react with cystatin A.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Cystatin B (stefin B, NCPI) is a natural inhibitor of lysosomal cysteine proteases (cathepsins). Its expression seems to be connected to the regulation of proteolysis which is essential for many physiological and pathological processes.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.