This antibody reacts with:
Synthetic peptide surrounding amino acid 322 of human cathepsins B
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry, Flow cytometry
Target of this antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-cathepsin B polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Alternative names of antibody target:
CTSB, CPSB, APPS, EC 220.127.116.11
Western blotting (0.5-4 µg/ml) amd in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The purified antibody detects both the proform (37-43 kDa) and the mature form (25 kDa) of cathepsin B. It reacts with human and mouse and in a lesser extent with rat samples.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy sμggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.