This antibody reacts with:
Synthetic peptide surrounding amino acid 514 of human Catalase
How to handle this antibody:
The antibody solution should be gently mixed before use.
Form of antibody:
This antibody has been succesfully tested for use in following methods:
Target of this antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
CAT, Cas1, Cast 1, cast-1
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 58 kDa protein, corresponding to the apparent molecular mass of Catalase on SDS-PAGE immunoblots, in samples from human origins.
Shelf time: 12 months in temp.: -20 deg.C
Clonality of antibody:
Catalase is known marker for peroxisomes. It is the most abundant protein in the peroxisomes. It is present in all aerobically respiring organisms. It protects cells form the toxicity of hydrogen peroxide.
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by biovision antibodies-kits-proteins they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.